Abstract -An Assessment of Stimulation of Hevea brasiliensis Rubber Biosynthesis by eIF-5A Protein-enriched Bacterial Lysates


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	Publications of MRB Latest Publications MRB Digest Journal of Rubber Research 3^rd Qtr 2008 Vol 11(3) 2^nd Qtr 2008 Vol 11(2) 1^st Qtr 2008 Vol 11(1) 4^th Qtr 2007 Vol 10(4) 3^rd Qtr 2007 Vol 10(3) 2^nd Qtr 2007 Vol 10(2) 1^st Qtr 2007 Vol 10(1) 1^st Qtr 2006 Vol 9(1) 2^nd Qtr 2006 Vol 9(2) 3^rd Qtr 2006 Vol 9(3) 4^th Qtr 2006 Vol 9(4) Submit paper/article Order Form	Abstract An Assessment of Stimulation of Hevea brasiliensis Rubber Biosynthesis by eIF-5A Protein-enriched Bacterial Lysates K.S. Chow#, Faridah Yusof, Salimah Mohd. Lim and Latifah Abdullah* The effect of six unique isoforms of Hevea brasiliensis eukaryotic translation initiation factor 5A (eIF-5A), previously reported as a rubber biosynthesis stimulator protein (RBSP) was evaluated using biosynthesis assays containing zone 2 rubber particles and radiolabelled isopentenyl diphosphate ([¹⁴C]-IPP). Recombinant RBSP proteins were over-expressed as N-terminal glutathione-S-transferase (GST)-tagged proteins in a bacterial system. Crude extracts of six RBSP-GST isoforms were added to rubber biosynthesis assays in increasing volumes. Results showed that increases in the amount of each RBSP-GST isoform corresponded with increases in the level of [¹⁴C]-IPP incorporation above that of the control which contained no recombinant protein. Statistical data analysis revealed that three RBSP isoforms increased [¹⁴C]-IPP incorporation significantly compared to the GST control where the RBSP isoform with the highest stimulatory effect produced a 45% increase in IPP incorporation compared to GST. back
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