Abstract - An Efficient Method for the Isolation of Good Quality RNA from Bark Tissues of Mature Rubber Trees


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	Publications of MRB Latest Publications MRB Digest Journal of Rubber Research 3^rd Qtr 2008 Vol 11(3) 2^nd Qtr 2008 Vol 11(2) 1^st Qtr 2008 Vol 11(1) 4^th Qtr 2007 Vol 10(4) 3^rd Qtr 2007 Vol 10(3) 2^nd Qtr 2007 Vol 10(2) 1^st Qtr 2007 Vol 10(1) 1^st Qtr 2006 Vol 9(1) 2^nd Qtr 2006 Vol 9(2) 3^rd Qtr 2006 Vol 9(3) 4^th Qtr 2006 Vol 9(4) Submit paper/article Order Form	Abstract An Efficient Method for the Isolation of Good Quality RNA from Bark Tissues of Mature Rubber Trees M.B. Mohamed Sathik, K.N. Nataraja, M. Thomas, J. Jacob* and M. Udayakumar In this study, an attempt has been made to extract good quality RNA from soft bark tissues of Hevea which generally contain plenty of interfering compounds such as phenolic compounds, tannins, lignins and carbohydrates. The method explained here involves removal of phenolic compounds using polyvinyl pyrrolidone and selective precipitation of RNA using lithium chloride. The purity of RNA prepared by this method was tested for its ability to get a good quality cDNA prepared. The cDNA produced from total RNA preparation was further confirmed by amplification of specific house keeping genes using quantitative real time PCR method. This protocol appears best suited for Hevea bark that is rich in several contaminating factors. back
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