Abstract - Relationship between in vitro Cell Culture Culture Cytotoxicity and Sweat-extractable Dithiocarbamates in Natural Rubber Latex Gloves


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	Publications of MRB Latest Publications MRB Digest Journal of Rubber Research 3^rd Qtr 2008 Vol 11(3) 2^nd Qtr 2008 Vol 11(2) 1^st Qtr 2008 Vol 11(1) 4^th Qtr 2007 Vol 10(4) 3^rd Qtr 2007 Vol 10(3) 2^nd Qtr 2007 Vol 10(2) 1^st Qtr 2007 Vol 10(1) 1^st Qtr 2006 Vol 9(1) 2^nd Qtr 2006 Vol 9(2) 3^rd Qtr 2006 Vol 9(3) 4^th Qtr 2006 Vol 9(4) Submit paper/article Order Form	Abstract Relationship between in vitro Cell Culture Cytotoxicity and Sweat-extractable Dithiocarbamates in Natural Rubber Latex Gloves Elizabeth K. Abraham, P. Ramesh, Roy Joseph, T.V. Kumari, P.V. Mohanan and V.M. Remakumari Cytotoxicity of some of the commercially available natural rubber latex gloves was evaluated by in vitro cell culture test using L929 mouse fibroblast cell lines. The cytotoxic potential ranged from non-cytotoxic to severely cytotoxic for the different brands tested. Since the cytotoxicity of latex products could be related to the release of leachable and toxic residual chemicals, the different brands of gloves were extracted in a physiologically simulated medium, namely, artificial sweat. The results revealed that a rubber accelerator, zinc diethyldithiocarbamate (ZDEC), was present in the gloves as residues and released into the artificial sweat. The amount of ZDEC released into the artificial sweat (sweat-extractable ZDEC) was estimated using high performance liquid chromatography. It was observed that there existed a linear relationship between the amount of sweat-extractable ZDEC and the cytotoxic potential of gloves represented in grades ranging from 0 to 3 as per ISO 10993-5 (1999) standard. This preliminary investigation showed that the amount of sweat-extractable ZDEC is a good indicator of the cytotoxicity potential of gloves and hence, the artificial sweat extraction may be further developed as a reliable test method to screen new latex medical products/formulations. back
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