Abstract - Isolation and Characterisation of Latex Cyanogenic Glucosidase in Hevea brasiliensis


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	Publications of MRB Latest Publications MRB Digest Journal of Rubber Research 3^rd Qtr 2008 Vol 11(3) 2^nd Qtr 2008 Vol 11(2) 1^st Qtr 2008 Vol 11(1) 4^th Qtr 2007 Vol 10(4) 3^rd Qtr 2007 Vol 10(3) 2^nd Qtr 2007 Vol 10(2) 1^st Qtr 2007 Vol 10(1) 1^st Qtr 2006 Vol 9(1) 2^nd Qtr 2006 Vol 9(2) 3^rd Qtr 2006 Vol 9(3) 4^th Qtr 2006 Vol 9(4) Submit paper/article Order Form	Abstract Isolation and Characterisation of Latex Cyanogenic Glucosidase in Hevea brasiliensis E. Sunderasan, M.A. Ward and H.Y. Yeang Cyanogenic glucosidases play an important role in defence against pathogen attack in plants. Hevea brasiliensis latex glucosidase was isolated from the latex B-serum by dialysis-induced protein precipitation and gel filtration. Following SDS polyacrylamide gel electrophoresis carried out under reducing conditions, the protein was observed to be about 57 kDa. Partial amino acid sequences of the protein matched published sequences of several plant glucosidases, including those of cyanogenic glucosidases. Enzyme assays revealed that the protein possessed glucosidase activity, this being confirmed by isozyme staining of the protein after electrophoretic separation on native polyacrylamide gels. Enzyme activity was also observed when linamarin was used as the specific substrate, confirming that the protein was a cyanogenic glucosidase (linamarase) as suggested by the partial amino acid sequences. The latex cyanogenic glucosidase is a component of an allergenic protein complex, Hev b 4. Cloning and sequencing of the complete cDNA encoding the glucosidase is ongoing. back
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