Abstract - Hev b 1, Hev b 2 and Hev b 3 Contents in Natural Rubber Latex and Powdered Latex Gloves


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	Publications of MRB Latest Publications MRB Digest Journal of Rubber Research 3^rd Qtr 2008 Vol 11(3) 2^nd Qtr 2008 Vol 11(2) 1^st Qtr 2008 Vol 11(1) 4^th Qtr 2007 Vol 10(4) 3^rd Qtr 2007 Vol 10(3) 2^nd Qtr 2007 Vol 10(2) 1^st Qtr 2007 Vol 10(1) 1^st Qtr 2006 Vol 9(1) 2^nd Qtr 2006 Vol 9(2) 3^rd Qtr 2006 Vol 9(3) 4^th Qtr 2006 Vol 9(4) Submit paper/article Order Form	Abstract Hev b 1, Hev b 2 and Hev b 3 Contents in Natural Rubber Latex and Powdered Latex Gloves H.Y. Yeang, C.H. Lau, Siti Arija M. Arif, Y.H. Loke, J.L. Chan, Samsidar Hamzah, R.G. Hamilton The contents of three latex allergens, Hev b 1, Hev b 2 and Hev b 3 were compared in 24 latex examination gloves and in fresh natural rubber latex using immunoassays that employed a two-site ELISA format. Hev b 1, Hev b 2 and Hev b 3 that were extractable from the powdered latex gloves were found to represent only a very small proportion of their contents in fresh latex and a very small proportion of the total extractable glove proteins. Of the three allergens studied, only Hev b 2 content was significantly correlated with total protein content. Hence, total protein content did not always reflect the contents of the individual latex allergens. Of the three allergens studied, Hev b 2 and Hev b 3 contents were significantly correlated with binding to IgE pooled from latex-allergic patients. One brand of glove had undetectable levels of the three individual latex allergens, indicating that it was feasible to remove a large proportion of the allergens during glove manufacture. back
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