Abstract - Expression of Human Serum Albumin in Transgenic Hevea Brasiliensis


	Journals and Publications


	Publications of MRB Latest Publications MRB Digest Journal of Rubber Research 3^rd Qtr 2008 Vol 11(3) 2^nd Qtr 2008 Vol 11(2) 1^st Qtr 2008 Vol 11(1) 4^th Qtr 2007 Vol 10(4) 3^rd Qtr 2007 Vol 10(3) 2^nd Qtr 2007 Vol 10(2) 1^st Qtr 2007 Vol 10(1) 1^st Qtr 2006 Vol 9(1) 2^nd Qtr 2006 Vol 9(2) 3^rd Qtr 2006 Vol 9(3) 4^th Qtr 2006 Vol 9(4) Submit paper/article Order Form	Abstract Expression of Human Serum Albumin in Transgenic Hevea Brasiliensis P. Arokiaraj, F. Ruker, E. Obermayr, A.R. Shamsul Bahri, J. Hafsah, D.C. Carter and H.Y. Yeang Transgenic rubber (Hevea brasiliensis) plants that secrete human serum albumin (HSA) in the serum fraction of rubber latex were generated, using a binary vector pLGMRHSA (13.8 kb) containing HSA cDNA (1.8 kb) with its native leader sequence in Agrobacterium tumefaciens (GV2260) containing a supervirulent plasmid (pToK47). The transformation success based on production of kanamycin-resistant callus, was calculated as 7.4%. Plant regeneration was 7.1 % based on production of embryoids selected on kanamycin containing medium. The frequency of successful establishment of these plantlets in soil was 22%, giving rise to 16 plantlets. These plantlets were screened for the presence of HSA cDNA (1.8 kb) and an internal fragment of nptll cDNA (0.7 kb) using the polymerase chain reaction. The presence of the recombinant protein was detected in leaf extracts of transgenic plants but not in controls. Similarly, the presence of the recombinant protein in latex of transgenic Hevea using a two-site ELISA was demonstrated. A production rate of 33 Ag HSA/mL of latex was achieved. Western blot analysis clearly demonstrated the presence of the 68 kDa protein in latex samples from transgenic plants. back
The Government and Malaysian Rubber Board shall not be liable for any loss or damage caused by the usage of any information obtained from this site. Malaysian Rubber Board 2002. All Rights Reserved. Best viewed with Internet Explorer 6.x browser.