| |
|
|
|
Abstract
Wound-induced Differentiation in Hevea Brasiliensis
Shoots Mediated by Jasmonic Acid
The effects of mechanical wounding on laticifer differentiation
in Hevea brasiliensis were investigated by using the young stems
in epicormic shoots. Light wounding by scraping to break the epidermal cells
of young stem usually caused the formation of a line of secondary laticifers
in the area roughly equal to that of the wound. More laticifers formed in
a larger area in response to heavier wounding, such as that caused by deep
scraping to remove part of cortex tissues of young stem or by cutting its
bark into xylem. The first laticifers induced by scraping and cutting were
visible as early as 6 days after wounding. The induction of laticifer differentiation
by wounding in young stem was enhanced by the application of jasmonic acid
and linolenic acid, whereas induction was hindered to different degrees
by the application of diethyldithiocarbamic acid and nordihydroguaiaretic
acid, which are inhibitors of jasmonate biosynthesis. Together with other
evidence, these observations indicate that laticifer differentiation upon
wounding is mediated by jasmonates. The involvement of jasmonates in wound
morphogenesis is discussed.

|