Abstract - The Use of Ultrafiltration for Removing Interferences in the Lowry Microassay of Extractable Proteins in Natural Rubber Latex Gloves


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	Publications of MRB Latest Publications MRB Digest Journal of Rubber Research 3^rd Qtr 2008 Vol 11(3) 2^nd Qtr 2008 Vol 11(2) 1^st Qtr 2008 Vol 11(1) 4^th Qtr 2007 Vol 10(4) 3^rd Qtr 2007 Vol 10(3) 2^nd Qtr 2007 Vol 10(2) 1^st Qtr 2007 Vol 10(1) 1^st Qtr 2006 Vol 9(1) 2^nd Qtr 2006 Vol 9(2) 3^rd Qtr 2006 Vol 9(3) 4^th Qtr 2006 Vol 9(4) Submit paper/article Order Form	Abstract The Use of Ultrafiltration for Removing Interferences in the Lowry Microassay of Extractable Proteins in Natural Rubber Latex Gloves This study evaluated the use of ultrafiltration in removing micro-solutes that might interfere in the Lowry microassay. Reference proteins, glove extracts and 1, 3-diphenylguanidine were subjected to ultrafiltration using a commercial concentrator, which has a membrane filter of 3 kD molecular weight cutoff. Separated fractions were analysed for their reactivity by the MS 1392: 1998 Lowry mircoassay. Purified reference proteins with molecular weights greater than 3 kD, showed high retentate recovery, which verified the efficacy of separation of the ultrafiltration unit. Micro-solutes were evidently present in both the non-interfering and interfering glove extracts. Irrespective of their original extractable protein (EP) values, the micro-solute content for the non-interfering glove extracts was relatively constant at 28%. This is in contrast with the marked variation observed for the interfering glove extracts. The ultrafiltration process caused an irrecoverable loss of approximately 5% - 6%. The total recovery was comparable for both the non-interfering and interfering glove samples. This indicates that the micro-solutes and/or their interfering components were not detrimental to the filter membrane, causing no obvious degradation of membrane integrity. Some samples may however, necessitate higher number of wash cycles to completely remove the interference and obtain accurate EP values.
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