Abstract - Structural characterisation of wild rubbers: Protein and ester content


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	Publications of MRB Latest Publications MRB Digest Journal of Rubber Research 3^rd Qtr 2008 Vol 11(3) 2^nd Qtr 2008 Vol 11(2) 1^st Qtr 2008 Vol 11(1) 4^th Qtr 2007 Vol 10(4) 3^rd Qtr 2007 Vol 10(3) 2^nd Qtr 2007 Vol 10(2) 1^st Qtr 2007 Vol 10(1) 1^st Qtr 2006 Vol 9(1) 2^nd Qtr 2006 Vol 9(2) 3^rd Qtr 2006 Vol 9(3) 4^th Qtr 2006 Vol 9(4) Submit paper/article Order Form	Abstract In vitro Interactions of Rubber Chemical Additives with Cells from Varying Lineages In cytotoxicity study, it is possible that in vitro cell responses will vary depending on the cell lineage. This consequently affects the toxicity ranking and the threshold levels considered safe for use. This study aims to examine the probable variations in the cytotoxic effects elicited by rubber chemical additives evaluated against three different cell lines, U937 (monocytic), HeLa (epithelial) and L929 (fibroblastic) cells. Additives tested were zinc diethyldithiocarbamate (ZDEC), zinc dibutyldithiocarbamate (ZBUD), zinc-2-mercaptobenzothiazole (ZMBT), zinc oxide (ZnO) and 2,2'-methylene-bis(4-methyl-6-tert-butylphenol) (2246). Cell response was quantified using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay, which measures the cell metabolic activity via the reduction of MTT salt by dehydrogenase enzymes in living cells. The lethal chemical concentrations (LC₅₀) against the three cell lines were constant for ZDEC and ZMBT, but varied for ZBUD, ZnO and 2246. This indicates cell dependent toxicity among some of the rubber chemical additives. Caution should therefore be exercised when using these toxicity threshold values to gauge the safety levels for use.
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