Abstract - Issues associated with using fluorescent microsperes to evaluate the barrier integrity of Natural rubber latex films


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	Publications of MRB Latest Publications MRB Digest Journal of Rubber Research 3^rd Qtr 2008 Vol 11(3) 2^nd Qtr 2008 Vol 11(2) 1^st Qtr 2008 Vol 11(1) 4^th Qtr 2007 Vol 10(4) 3^rd Qtr 2007 Vol 10(3) 2^nd Qtr 2007 Vol 10(2) 1^st Qtr 2007 Vol 10(1) 1^st Qtr 2006 Vol 9(1) 2^nd Qtr 2006 Vol 9(2) 3^rd Qtr 2006 Vol 9(3) 4^th Qtr 2006 Vol 9(4) Submit paper/article Order Form	Abstract Issues Associated with Using Fluorescent Microsperes to Evaluate the Barrier Integrity of Natural Rubber Latex Films It has been reported that 100nm – 110 nm fluorescent microspheres can pass through more natural rubber (NR) latex condoms than can the 27nn virus ΦX174 as found by other investigators. Are there properties of fluorescent polystyrene microspheres that could be responsible for these disparate results? This study demonstrated that the microspheres adsorbed to a brand of condom made of NR latex as well as a highly-adsorptive virus, PRDI (ΦX174 did not adsorb). The study also found that free fluorescent dye was not released when the microspheres were in close contact with the NR latex. These findings argue against microsphere properties being responsible for the disparate results. In addition, an increase in the fluorescence of buffer after contact with NR latex condom was found, in the absence of microspheres. This increase may require extended contact. Thus, this study found an artifact that could be misconstrued as evidence of fluorescent microspheres passing through NR latex barriers.
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